SP analysis of subsets defined by SCA-1 and CD11b expression. Analysis of a representative murine marrow that was Hoechst stained and labeled for SCA-1 and CD11b. Subsets defined by SCA-1 and CD11b expression (B) were displayed on Hoechst red/blue dotplots (dotted line indicates level of matched isotype control). SCA-1⁺/CD11b^lowcells were more enriched for SP (D; 3.25%), than SCA-1^neg/CD11b⁺ (C; 0.017%) or whole BM (A; 0.1%).