Generation of large number of IPCs from adult CD34⁺ HPCs obtained from peripheral blood of G-CSF–mobilized donors. Purified CD34⁺ HPCs from peripheral blood of G-CSF–mobilized donors were cultured in medium supplemented with FLT3-L or TPO alone or FLT3-L and TPO (50 ng/mL). Cultured cells were counted and analyzed for HLADR⁺CD11c^-CD123^high IPCs, HLADR⁺CD11c⁺CD123^dim immature DCs, CD14⁺ monocytes, and CD34⁺ HPCs on days 10, 15, 20, 25, and 30. (A) Total cell yields from 10⁶ HPCs cultured in medium with FLT3-L, TPO, or FLT3-L/TPO. Data shown are aggregate results of experiments with HPCs from 4 donors. (B). Total cell yields from 10⁶ HPCs cultured in medium with FLT3-L/TPO. Data shown are aggregate results of experiments with HPCs from 6 donors. (C) IPCs and CD11c⁺ immature DCs and their frequencies (%) in day-30 HPC culture with FLT3-L/TPO. (D) CD14⁺ monocytes and CD34⁺ cells and their frequencies (%) in day-30 HPC culture with FLT3-L/TPO. (E) Day-30 cell yields of IPCs, CD11c⁺ immature DCs, CD14⁺ monocytes, and CD34⁺ cells under 3 different culture conditions of the experiments in panel A. ^*P < .01 (compared day-30 yield of each cell population from FLT3-L/TPO cultures with FLT3-L or TPO culture alone). (F) Kinetics of cell yields of IPCs, CD11c⁺ immature DCs, CD14⁺ monocytes, and CD34⁺ cells from 10⁶ CD34⁺ HPCs of experiments in panel B. Data presented in panels E (n = 4) and F (n = 6) are aggregate results and are expressed as means ± SD.