IPCs derived from HPCs in FLT3-L/TPO cultures exhibit morphology typical of and TLR-9 expression similar to those of peripheral blood IPCs. (A) Giemsa staining of IPCs purified from day 25 HPC culture in FLT3-L/TPO was examined under a Nikon E1000 microscope at × 1000 original magnification. (B) Sorted IPCs and CD11c⁺ immature DCs from day 25 culture of HPCs in FLT3-L/TPO were assessed for their expression of TLR1, TLR3, and TLR9, as detailed in “Materials and methods.” Data shown are representative of 3 experiments.