Death induced by grB and AdV, but not by staurosporine, is dependent on dynamin. HeLa-DynWT or DN cells were treated to suppress (+tet) or induce (–tet) overexpression from the transfected dynamin cDNA. Caspase activation was assessed by labeling with M30 mAb. The number of M30-positive cells was quantified by flow cytometry. (A) Cells were treated with grB and AdV for 2 hours at 37° C. (B) Cells were treated with staurosporine (STS) for 2 hours at 37° C. The mean ± SD of 4 independent experiments is shown (***P < .001).