Increased number of microvessels in K14-AGF transgenic mice. (A) Gross appearance of the K14-AGF mouse and a control showing that the skin of ears and snout of K14-AGF mice are red compared with controls. (B) Immunohistochemical analysis with anti–PECAM-1 antibody of ear skin from K14-AGF mouse and a control. Increased PECAM-1⁺ microvessels (purple) are detected in the dermis and subcutaneous layers of K14-AGF mouse. Bar indicates 100 μm. (C) Electron microscopic analysis (original magnification, × 1700) shows that increased vessels are capillary-sized (arrows) in the K14-AGF mouse. BC indicates epidermal basal cells. (D-E) Representative photograph of blood vessels in the ear from the K14-AGF mouse (D) and controls (E) stained with fluorescein-labeled Lycopersicon esculentum lectin. Abundant capillary-sized vessels in the K14-AGF mouse are detected. (F-G) Quantitative analysis for the number of vessels shown in panels D and E. Length of vessels (F) and number of vessel joints (G) in K14-AGF transgenic mice (▪) relative to controls (□) are shown as percentages. Columns represent mean values + SD (n = 5).