AGF induction of vascular leakage. For a vascular permeability assay, male 8-week-old BALB/c mice were anesthetized, and 100 μL 1% Evans blue dye was injected into the tail vein. At 5 minutes after injection of dye, AGF (1 μg or 3 μg), ARP4 (3 μg), VEGF (0.1 μg), or PBS was injected intradermally at adjacent locations on the back skin. (A) A representative result of dye leakage in the back skin 30 minutes after injection of AGF (3 μg, left) and PBS (right) is shown. Original magnification, × 8.4. (B) Vascular leakage is measured spectrophotometrically by Evans blue content of back skin 30 minutes after intradermal injection. Columns represent mean values ± SD (n = 5).