AGF induces in vivo neovascularization. AGF induces angiogenesis in an in vivo Matrigel assay (A-I). (A) Matrigel containing AGF (left) or PBS only (right) was subcutaneously injected near the abdominal midline of 8-week-old C57BL/6 mice. Gross appearance of Matrigels on day 5. (B-G) Histologic analysis of sections from Matrigels. Cells in Matrigels were visualized by staining with May-Giemsa (B-E). Endothelial cells were visualized by staining with anti–PECAM-1 antibody (F-G). Scale bars indicate 100 μm. (H) Black (Matrigel containing AGF) and white (Matrigel only) columns represent the area of vessels, which was measured quantitatively by means of an image analyzer. The data are presented as mean values ± SD (n = 5). (I) Values represent the concentration of hemoglobin per Matrigel plug weight ± SD for 5 assays. (J-K) AGF induces angiogenesis in a corneal neovascularization assay (n = 5). Gross appearance of neovascularization in the cornea. Pellets containing AGF (J) induced neovascularization, while pellets containing control buffer (K) did not.