G-CSF stimulates the formation of CFU-G colonies in mut10-transduced BM cells but not in BM in which Cebpa has been excised. To determine the differentiation potential of mut10-transduced cells in response to G-CSF alone, Lin^neg BM cells from wild-type B6 mice were infected with either FMEV-mut10 or control vector FMEV-GFP, sorted for GFP expression, and then plated in methylcellulose containing G-CSF. As a control, Lin^neg BM were isolated from SWB mice homozygous for floxed Cebpa alleles. BM cells were infected with either FMEV-CRE or FMEV-GFP, sorted for GFP expression, and plated in G-CSF. Colonies were scored 7 days after plating. Results presented are from a single experiment performed in triplicate. Error bars indicate SEM.