Figure 3.
FMEV/mut10-infected cells still contain a high percentage of granulopoiesis after 14 days in culture. Flow cytometric profiles of cells from FMEV-mut10 (A) or control FMEV-eGFP (B) cultures 14 days after infection and maintenance in SCF, IL3, FLT3, and IL11. The percentage of cells double-positive for CD11b and Gr1 is indicated for each culture, as well as the mean fluorescence intensity of Gr1, of which the expression level correlates with granulocytic maturation. Double-negative cells were c-kit positive and confirmed to be mast cells by microscopic examination of cytospins.