Fusion of FIP1L1 to PDGFRA in the EOL-1 cell line. Detection of the FIP1L1-PDGFRA fusion transcript in the EOL-1 and EOL-3 cell lines (A), detection of FIP1L1 expression in the EOL-1 cell line (B), and amplification of the FIP1L1-PDGFRA fusion gene on DNA from the EOL-1 cell line (C). Different transcripts are observed for FIP1L1 and FIP1L1-PDGFRA (A-B) because of alternative splicing. Expressions of PDGFRA-FIP1L1 and native PDGFRA were not detected (B). The sequence of the fusion gene surrounding the deletion is shown at the DNA level, at the RNA level (after splicing), and at the protein level (D). Splice donor and acceptor sites are underlined. A cryptic splice site is used in exon 12 of PDGFRA. Schematic representation of FIP1L1, PDGFRα, and FIP1L1-PDGFRα proteins (E). Points at which the proteins are interrupted by the deletion are indicated by arrowheads.