Methylation status of DNA fragments isolated by MCA/RDA. Genomic DNA was treated by sodium bisulfite and amplified by primers specific for DNA regions identified by MCA/RDA. Then, PCR products were subcloned into plasmid DNA, and the sequences were determined in 10 clones of each gene: (A) MEL1, (B) CACNA1H, and (C) Nogo receptor gene. The schemas show the structures of isolated DNA regions (bold bars), and arrowheads indicate the SmaI/XmaI sites. The methylation status of each CpG site is shown (•, methylated CpG; ○, unmethylated CpG).