Expression of genes isolated by MCA/RDA in various cell lines. Expression of the MEL1, CACNA1H, and Nogo receptor genes, as identified by MCA/RDA, was analyzed by RT-PCR. Transcripts of the GAPDH gene were used as a control. Lane 1, 293 cells; lane 2, PBMCs; lane 3, activated T cells; lane 4, MT-4; lane 5, ATL-35T; lane 6, ATL-43T; lane 7, ATL-55T; lane 8, ED; lane 9, ATL-2; lane 10, Hut78; lane 11, Jurkat; lane 12, SupT1; and lane 13, Kit225.