Correlation between quantitative parameters of HIV and HCV infection in macrophage cultures. (A) Amplification of synthetic HIV RNA template with real-time quantitative RT-PCR: the assay detected 100 template copies and remained linear up to 109 template copies. (B-D) Relationship between HCV RNA cellular load at 2 weeks and HIV RNA quantity in culture supernatant at time 0 (B; right before exposure to HCV), 7 days (C), and 14 days (D) after HCV exposure. There was significant correlation (r = 0.96; P < .01) for HCV RNA and HIV RNA quantity at time 0. (E) Relationship between quantity of HCV RNA and proviral HIV DNA in cells at 2 weeks. (F) Relationship between the quantity of HCV RNA in cells and the percent of cells expressing p24 at 2 weeks. All correlations were calculated by nonparametric Spearman rank test.
