Figure 2.
CCL25/TECK-induced calcium mobilization on CCR9+ T-ALL blasts. The human chemokines CCL25/TECK (CCR9 ligand) and CXCL12/SDF-1α (CXCR4 ligand) were tested for calcium mobilization on 2-mL aliquots of 107/mL Fluo-3 AM-loaded T-ALL blasts. The left panel shows the dose-response of patient 7125 T-ALL blasts to human CCL25/TECK (hCCL25/TECK) and the response to hCXCL12/SDF-1α; the right panel shows the response of patient 5232 to these chemokines. The chemokine receptor expression is indicated at the top of each panel. Ionomycin was also used as a stimulator of calcium influx to obtain the maximal response. The arrows indicate the time of additions.
