Detection of C1q⁺ and C1q^– DCs in human tonsils. Cryostat tonsil sections were analyzed for DC markers and C1q through single staining. Sections were incubated with rabbit antihuman C1q (A; original magnification, × 100) (B; original magnification, × 400), mouse antihuman DC-SIGN (C; original magnification, × 100), and mouse antihuman DC-LAMP (D; original magnification, × 100), as described in “Materials and methods.” Cryostat tonsil sections were also analyzed through double staining (E-H; original magnifications, × 630). Sections were incubated with mouse antihuman DC-SIGN and rabbit antihuman C1q (E-F, respectively) or mouse antihuman DC-LAMP and rabbit antihuman C1q (G-H, respectively). B indicates B-cell follicle; T, T-cell zone.