Dysregulated megakaryopoiesis in V205GR mice. (A) CD41 expression in bone marrow cells from wild-type (left panels) and V205GR (right panels) mouse. In the total ungated population, 30 000 events were acquired (upper panels). The lower panels show the histogram of CD41⁺ cells in the gated population of cells shown in the corresponding upper panels. (B,C) Megakaryocyte colonies from wild-type (B) and V205GR (C) bone marrow mononuclear cells. Colony formation assay was performed as described in the text, and megakaryocyte colonies are visualized by AChE staining. Original magnification, × 100.