Optimization of the number of DCs for enhancement of CNAR. Monocytes were cultured with GM-CSF and IL-4 for 3 days and matured with CD40L trimer. In a 96-well plate, DCs at 3-fold dilutions were then added to 50 000 CD8⁺ cells/well. After 6 days of coculture, the CD8⁺ cells were removed and transferred into a coculture with HIV-1-infected CD4⁺ cells. Supernatants from duplicate cultures were assayed for RT activity.²⁶  The extent of CNAR (▦) was determined by comparing these RT results with the average RT activity in supernatants of HIV-infected CD4⁺ cells cultured alone. Cell proliferation (▧) was measured as described in “Patients, materials, and methods.” Error bars indicate SD from the average suppression observed. Results are representative of 5 separate experiments.