RT-PCR of primary hematopoietic cell populations for EPAC1 and 2. (A) RT-PCR for EPAC1 of cDNA derived from B-CLL, T-CLL, ALL, and mantle-cell lymphoma cells. As a control, cDNA was also amplified for actin. (B) Same RT-PCR analysis as for panel A except that cDNA were derived from purified human B cells, T cells, neutrophils, and monocytes. (C) Same analysis as for panels A and B but using oligonucleotides specific for amplification of human EPAC2. The cDNA from HEK-293 cells was used as a positive control.