Apoptosis analysis of B-CLL cells following treatment with agents that activate both EPAC and PKA or EPAC alone. Apoptosis was determined by Hoechst 33342 flow cytometry in B-CLL cells incubated for 48 hours with 8CPT-2Me-cAMP, 10 μM rolipram and 40 μM forskolin (Roli/Fsk), 200 μM 8-Bromo cAMP, 200 μM N6-benzoyl cAMP, 1 mM (R_p)-8-Br-cAMPS, or combinations of these drugs, as indicated. The 8CPT-2Me-cAMP concentrations are given in μM, where indicated; in patients 1 and 3, the drug was used at 50 μM. The SEM of triplicate samples is shown.