Expression of AHI-1 isoforms are variably altered in human leukemic cell lines. (A) Schematic diagram of human AHI-1 isoforms (i-iii). The numbers refer to the positions of the nucleotides. Open reading frames are indicated by thick bars, boxes, and triangles. Untranslated sequences are indicated by thin lines. In-frame stop codons are indicated by asterisks. The position of the specific primers used to detect each isoform are indicated by the arrows. (B) Absolute AHI-1 isoform transcript levels were calculated as described in “Patients, materials, and methods” from real-time RT-PCR analyses of a single set of the same RNA samples used to generate the data shown in Figure 3A but, in this case, specific primers to detect each isoform separately (A) and the corresponding standard curves were used. Lane 1 indicates unfractionated normal BM cells; lanes 2-9, various myeloid cell lines known as Mo7E, AML5, KG1, TF1, U937, HL60, K562, THP1; lanes 10-17, various lymphoid cell lines known as Im9, Daudi, Raji, Jurkat, CC119, MT4, Hut102, and Hut78.