Enhanced expression of AHI-1 in primary CML cells. (A) Absolute AHI-1 transcript levels were calculated as described in “Patients, materials, and methods” from real-time RT-PCR analyses of RNA isolated from FACS-purified lin^–CD34⁺CD38^–, lin^–CD34⁺CD38⁺, lin^–CD34⁺, and lin⁺CD34^– normal BM (n = 4) and CML cells in chronic phase (n = 18), accelerated phase (n = 8), and myeloid blast crisis (n = 2). The mean levels of AHI-1 expression in each population are indicated by the horizontal bars. (B) A similar analysis performed on RNA isolated from later subsets of lin⁺ normal human BM cells (n = 4) and chronic-phase CML cells (n = 6). (C) Real-time RT-PCR analyses of RNA isolated from FACS-purified lin^–CD34⁺CD38^– normal BM (n = 4) and chronic-phase CML cells (n = 8) using the same isoform-specific primers shown in Figure 4A and the corresponding standard curves. Values shown are the mean ± SEM.