Increased expression of AHI-1 in quiescent versus cycling CD34⁺ CML cells. (A) Representative examples of Hst and Py staining of viable (PI^–) CD34⁺ cells from normal human BM (left) and from a CML sample (right). (B) Absolute AHI-1 transcript levels calculated as described in “Patients, materials, and methods” from real-time RT-PCR analyses of RNA isolated from G₀ and G₁/S/G₂/M fractions of normal BM CD34⁺ cells (n = 3) and CD34⁺ cells from chronic-phase CML samples (n = 6). Values shown are the mean ± SEM.