Aurora-B/AIM-1 expression in primary megakaryocytes at interphase. Mouse primary megakaryocytes were stained with DAPI (blue; i), anti–Aurora-B/AIM-1 antibody (red; ii), or FITC-conjugated anti-CD41 (green; iii). Triple staining (iv). Shown are representative images captured as described in “Materials and methods.” (A) Mature megakaryocyte (not cycling) with ploidy of 16N at interphase. (B) Polyploidizing megakaryocyte with ploidy of 8N at interphase/early prophase. (C) Polyploidizing megakaryocyte with ploidy of 32N at interphase/early prophase. Original magnification, × 1500.