Role of PAF and its receptors on VEGFA₁₆₅–induced P-selectin translocation. HUVECs were pretreated with selective PAFR antagonists, at the indicated concentrations, for 15 minutes prior to stimulation with VEGF-A₁₆₅ (10^–9 M, 7.5 minutes; A) or PAF (10^–9 M, 7.5 minutes; B). Pretreatment with intracellular (LAU 8080), extracellular (BN 52021) and nonspecific PAFR (CV-3988) antagonists reduced significantly the translocation of P-selectin induced by VEGF-A₁₆₅ and PAF. Data are means ± SEM of at least 6 experiments, *P ≤ .05 and ***P ≤ .001 as compared to DPBS, †††P ≤ .001 compared to VEGF-A₁₆₅ (A) or PAF (B).