Averaged gel comparison of Lin^–Sca⁺Kit⁺ and Lin^–Sca⁺Kit^– cells labeled with saturation dyes. The use of averaged gels to allow sample comparison for Lin^–Sca⁺Kit⁺ and Lin^–Sca⁺Kit^– cell lysates. Data from 5 sets of reciprocally labeled Lin^–Sca⁺Kit^–/+ populations were pooled and significant differences in protein expression (normalized volume) calculated using a 2-tailed Student t test. (A) All protein spots common to both gels are shown with enclosed areas. (B) Representative Lin^–Sca⁺Kit^– gels showing spots unique to Lin^–Sca⁺Kit^–. (C) Spots unique to Lin^–Sca⁺Kit⁺.