Figure 2.
Figure 2. Endothelial cell Ang-2 is localized in Weibel-Palade bodies. (A) Colocalization of endogenous Ang-2 (detected by Tie-2-Fc) and VWF in HUVECs. (B) Colocalization of Ang-2 and VWF in HUAECs, HAoECs, and HSaVECs. (C) Ultrastructural double immunoelectron microscopy of Ang-2 (large 10-nm particles) and VWF (small 6-nm particles) demonstrating colocalization of Ang-2 and VWF. The poor Weibel-Palade body morphology is due to the fixation procedure that imposes limitations on the postembedding immunocytochemistry. (D) VWF is required for Ang-2 storage in Weibel-Palade bodies (arrows). Cells overexpressing Ang-2 but not VWF express Ang-2 in a uniform cytoplasmic pattern (asterisks). Scale bar in panels A and D is 20 μm (panel B set to the same scale as panel A); in panel C, the original magnification is × 45 000.

Endothelial cell Ang-2 is localized in Weibel-Palade bodies. (A) Colocalization of endogenous Ang-2 (detected by Tie-2-Fc) and VWF in HUVECs. (B) Colocalization of Ang-2 and VWF in HUAECs, HAoECs, and HSaVECs. (C) Ultrastructural double immunoelectron microscopy of Ang-2 (large 10-nm particles) and VWF (small 6-nm particles) demonstrating colocalization of Ang-2 and VWF. The poor Weibel-Palade body morphology is due to the fixation procedure that imposes limitations on the postembedding immunocytochemistry. (D) VWF is required for Ang-2 storage in Weibel-Palade bodies (arrows). Cells overexpressing Ang-2 but not VWF express Ang-2 in a uniform cytoplasmic pattern (asterisks). Scale bar in panels A and D is 20 μm (panel B set to the same scale as panel A); in panel C, the original magnification is × 45 000.

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