Exclusion of Ang-2 and P-selectin storage in endothelial cell Weibel-Palade bodies. P-selectin (A) and Ang-2 (B) are both expressed in a distinct granular pattern in endothelial cells. Yet, their expression does not overlap (C). (D-F) Granular storage of P-selectin and Ang-2 is not just mutually exclusive in different cells, but also in Weibel-Palade bodies of the same cell (panel D, P-selectin; panel E, Ang-2; and panel F, double staining of P-selectin and Ang-2 with nonoverlapping expression in red and green granules). Scale bar is 20 μm.