Figure 1.
GO cytotoxicity can be measured in multiparameter flow cytometry assays. After drug treatments, TF1 cells were exposed to the potentiometric dye DiOC6(3) and the DNA dye propidium iodide (PI) that were used to identify apoptotic cells with reduced mitochondrial membrane potential (low DiOC6(3) staining) and dead cells with absent plasma membrane integrity (high PI staining), respectively, so that live (not apoptotic, not dead) cell fractions could be assessed. The GO-sensitizing activity of PK11195 was measured as further decreased live cell fractions in GO plus PK11195 cotreated cells. ψ indicates membrane potential.
