Figure 4.
FACS analysis of marrow cells isolated from NOD/SCID mice 7 weeks after injection of human cell grafts. Cells were stained for human CD45, CD19, CD41, and CD34 to assess multilineage engraftment. (A-C) Analysis of a mouse (n = 1) that did not receive any human cell graft (negative control). (D-F) Analysis of the marrow from mice (n = 4) receiving cells exposed to cytokines alone. (G-I) Analysis of the marrow from mice (n = 3) receiving cells exposed to 5azaD and TSA. (A) Isotype negative control. (B) CD19 PE and CD45 FITC negative control. (C) CD34 PE and CD45 FITC negative control. (D) Isotype cytokines alone. (E) CD19 PE and CD45 FITC cytokines alone. (F) CD34 PE and CD45 FITC exposed to cytokines alone. (G) Isotype exposed to 5azaD and TSA. (H) CD19 PE and CD45 FITC of cells exposed to 5azaD and TSA. (I) CD34 PE and CD45 FITC exposed to 5azaD and TSA.