Reconstitution of rhesus macaques with autologous, genetically modified hematopoietic cells. Various cell populations were gated based on forward and side scatter properties using FACS. The percentages of GFP-positive granulocytes (▴), monocytes (○), lymphoid cells (▪), red cells (□), and platelets (▵) at various times after infusion of transduced, autologous CD34⁺ cells are shown. The vector copy number in DNA from peripheral blood leukocytes was estimated by real-time PCR (•, heavy line) using DNA from a K562 erythroleukemia cell clone having a single copy of the vector genome as a standard. Data are from the following animals: (A) CJ5B, (B) RQ2617, and (C) RQ3556.