Kinetics of saturation of RhD-positive RBCs with anti-RhD IgG according to the dose of MonoRho and Rhophylac at 1500 IU (300 μg). RhD-positive RBCs (15 mL) were administered 24 hours prior to the anti-RhD injection. Saturation of RBCs at the indicated time points was measured using a FACS assay on samples treated without and with additional anti-RhD treatment (to allow for saturation of binding) during staining.