FISH detection of trisomy 8⁺cells produced in mice that received transplants of MDS cells. Panel A shows a cytospin of starting cells from patient 9, and panel B shows some of the human CD45/71⁺ cells isolated by FACS from the BM of a mouse injected 3 weeks previously with these cells. (C-D) Examples of cells from a mouse injected 3 and 5 weeks previously with cells from patient 1. Trisomy 8⁺ cells were identified by the detection of 3 fluorescent signals in single nuclei. Original magnifications, × 100 for all panels.