Tracking of individual clones by way of LAM-PCR and conventional PCR. (A) Gene Scan results of LAM-PCR. DNA (100 ng) isolated from 95E003 granulocytes before, during, and after G-CSF plus SCF treatment show the presence and the absence of clone no. 398. (B) Conventional PCR for clone no. 398 using clone-specific primers. DNA (100 ng) was used for nested PCR analysis using no. 398 specific primers (SP1, SP2) with an LTR primer. Note the presence of no. 398 clone before and after cytokine treatment. DNA from an animal (RQ2265) that received transduced cells with the same vector was used as a negative control.