Frequency of detections for randomly selected clones. (A) For 9 randomly selected representative clones from animal 95E003, the number of times the clone was detected of a total of 18 LAM-PCR reactions (6 replicates in 3 independent experiments) for each time point is shown. Each panel shows detections for an individual clone, and the clone designation (size of LAM-PCR product encompassing the shared LTR sequence and the genomic insertion through the next Tas1 site, in base pairs) is given in the right-hand upper corner of each panel. The periods of cytokine administration are indicated by the solid horizontal lines, first G-CSF alone, then G-CSF plus SCF. These typical patterns show the stochastic variation seen in the relative level of contributions from each clone and the lack of significant effect of either G-CSF alone or G-CSF plus SCF on these patterns. Clones such as 318 that contribute at a relatively low level (infrequent detections) continue in this pattern during and following cytokines, whereas clones with more frequent detections overall (clone 149) continue to contribute at this level during and following cytokine administration. (B) Frequency of detection of 9 randomly selected clones from animal RQ2265. Overall, the level of marking and frequency of detection of clones in this animal were lower, but the cytokines (G-CSF and SCF, administration shown as solid horizontal bar) again did not change the overall pattern of the frequency of clone detection.