Thymocyte and signal joint T-cell excision circle (sjTREC) reconstitution. Thymocytes were harvested and counted 42 days after congenic stem cell transplantation. sjTRECs were determined by quantitative real-time PCR. The values represent mean ± SD from 3 to 6 animals per group. (A) Total thymocytes. P < .05 except 5000 KTLS versus normals and normal CD45.1 versus normal CD45.2. (B) Total sjTRECs per thymus. P < .05, 400 KTLS versus 5000 KTLS; P = .1, 1000 KTLS versus 400 KTLS and 5000 KTLS. (C) Percentage of thymocyte subsets. P = not significant (NS) between transplantation recipients except 400 KTLS versus 1000 KTLS in percent CD4-8-; P < .05, transplantation recipients versus normals. (D) Frequency of sjTRECs in thymocytes. P < .05, normal CD45.1 versus 1000 KTLS and 5000 KTLS. KTLS indicates c-Kit+Thy1.1lowLin-lowSca-1+ hematopoietic stem cells; CD45.1 mice, C57BL/Ka, CD45.1, Thy1.1 mice; and CD45.2 mice, C57BL/6, CD45.2, Thy1.2 mice.
