GVL anti-mCP-CML experimental design. (A) Retroviral construct (Mp210/NGFR). LTR indicates long terminal repeat; IRES, internal ribosome entry site. (B) GVL against mCP-CML model. On day -6, mice were injected intravenously with 5 mg 5-FU, and after 4 days BM was isolated and prestimulated (day -2) with SCF, IL-3, and IL-6. Cells underwent spin infection with Mp210/NGFR retrovirus on days -1 and 0. Cells were infused into irradiated syngeneic wild-type recipients along with T-cell-depleted (TCD) BM from allogeneic donors, with or without donor T cells. (C) Representative flow cytometry of peripheral blood from a mouse with mCP-CML.