Lymphoid reconstitution in HSC chimeras. FL-derived HSCs were transferred into alymphoid mice (Rag°g°_c) to generate hematopoietic chimeras as described in “Materials and methods.” (A) Flow cytometric analysis of splenocytes stained for B (CD19⁺) and T (CD3⁺) cells. NK cells (CD122⁺DX5⁺) were detected in the CD19^-CD3^- fraction. (B) Absolute numbers of NK cells (CD3^-CD122⁺DX5⁺) in WT (•) and IκBα°ϵ° () chimeras. Each circle represents values obtained from a single mouse and a gray bar indicates the mean values. There is a significant decrease in BM, liver, and splenic NK cells (P < .001) in the absence of IκBα and IκBϵ compared with controls.