Clonogenic assays of liver and peripheral blood reveal differences between SHIP^-/- and Pten^+/-SHIP^-/- myeloid progenitors. (A) Splenocytes plated in duplicate revealed no differences in splenic CFUs between SHIP^-/- and Pten^+/-SHIP^-/-, but they did show an increase in the granulocyte/macrophage lineages over wild-type and Pten^+/-cells. (B) Liver cells demonstrated a significant increase in total CFUs (predominantly granulocytic and macrophage) in cells from Pten^+/-SHIP^-/- mice. (C) Colony-forming assays performed on peripheral blood revealed a statistically significant increase in SHIP^-/- CFUs that was not seen in Pten^+/-SHIP^-/- peripheral blood samples. ^***P < .05 compared with all genotypes; ^**P < .01 compared with wild-type and Pten^+/- only. ^*P < .025 compared with wild-type alone. Data were compiled from at least 3 mice per genotype plus or minus SEM of the number of colonies.