mtDNA mutations and clonal expansions of individual CD34 clones. (A) Reverse-sequence analysis of BM CD34 (clone number 2-6), PB CD34 (clone number 1-2), and single granulocyte (number g1-108) mtDNA from donor 4 showed the same poly C length heteroplasmy (mixture of 8CT6C and 9CT6C) between nucleotide position 303 to 315. Reverse-sequence analysis after TA cloning revealed poly C length heteroplasmy of 8CT6C (bottom left) and 9CT6C (bottom right). (B) Clonal expansion of substitution mutation at mtDNA nucleotide position 264. Sequence chromatogram with aggregate genotype (i) and 264C>T mutation was found in BM CD34 clone (number 1-6) (ii), PB CD34 clone (number 2-1) (iii), and single granulocyte (number g1-106) (iv) from donor 5.