ADAMTS13 fragments used for epitope mapping. (A) Domain organization of ADAMTS13 and a map of the ADAMTS13 fragments (I, II, III, IV, V, and VI) expressed in E coli. Indicated are the signal peptide (S), the propeptide (P), the catalytic, disintegrin, cys-rich, and spacer domains, the 2 CUB domains, and the 8 TSP type 1 repeats (1-8) (according to Zheng et al¹¹ ). (B) Silver staining of purified recombinant ADAMTS13 fragments expressed in E coli (a-c). Contaminating bands derived from E coli that could not be removed during the purification process. (C) Western blot analysis of the purified recombinant ADAMTS13 fragments using an anti-histag antibody. (D) Western blot analysis of the purified recombinant ADAMTS13 fragments using a polyclonal rabbit antihuman ADAMTS13-his antiserum. Fragment I indicates propeptide; fragment II, tsp1-1; fragment III, cat/dis/tsp1-1; fragment IV, cys-rich/spacer; fragment V, tsp1/2-8; fragment VI, cub1 + 2.