Reversion of the T-cell repertoire to normal in 3 HAA patients after immunosuppressive therapy. Samples for case no. 1 and no. 2 were collected at 1 year after immunosuppressive treatment and for case no. 3 at 2.5 years (antithymocyte globulin [ATG], cyclosporine [CsA], and mycophenolate mofetil [MMF]). Shown are the CDR3 length (40 amino acids) in the x-axis versus the fluorescence intensity in the y-axis. The spectratype profiles of the 21 V_β subfamilies were analyzed in 3 ways: observation, measuring the relative fluorescence intensity, and complexity scoring. Absent indicates no signal detected with the use of the current technique; skewed spectratype (observation), the percentage of non-Gaussian spectratypes as determined by eye; skewed (RI), the percentage of skewed spectratypes detected by means of a calculation based on the relative fluorescence intensity of each peak; total complexity score, the total number of discrete peaks in each V_β subfamily, as a measure of the polyclonality of the T-cell repertoire.