Recombinant PRCP. Schneider insect (S2) cells were transfected with either 19 μg pMT/BiP/V5-His C (empty vector) or 19 μg rPRCP-pMT/BiP/V5-His C (rPRCP). The full-length rPRCP was purified from serum-free medium as shown by colloidal Coomassie blue-stained SDS-PAGE (A) or immunoblot with goat antibody to PRCP (B).