Laser scanning confocal microscopy of HUVEC PRCP. PRCP is shown in red and LAMP1 in green. HUVECs were grown on microscope slides for 2 hours and then fixed with 2% paraformaldehyde. Nonpermeabilized or permeabilized fixed cells were incubated with goat anti-PRCP and mouse anti-LAMP1 for 1 hour at 37°C. After washing, the PRCP and LAMP1 antigens were detected with a secondary antibody labeled with Alexa Fluor 594-labeled donkey antigoat antibody and FITC-conjugated sheep antimouse antibody, respectively, as described in “Materials and methods.” Original magnification × 60.