Colocalization of PRCP with HK receptors. HUVECs were grown on microscope slides for 2 hours and then fixed with 2% paraformaldehyde and not permeabilized. Fixed cells were then incubated with goat anti-PRCP and anti-gC1qR, anti-uPAR, or anti-CK1 for 1 hour at 37°C. After washing, the anti-PRCP antibody was detected with Alexa Fluor 594-labeled secondary antibodies. Anti-gC1qR, -uPAR, and -CK1 antibodies were detected with FITC-conjugated secondary antibodies, as described in “Materials and methods.” Original magnification × 60.