Expression of annexin II in human leukemia cell lines. (Top 2 panels) Northern blot analysis of poly(A)⁺ RNA (1 μg per lane) isolated from human leukemia cell lines. The blot was hybridized with an annexin II cDNA probe and then rehybridized with a β-actin probe. (Lower 3 panels) Immunoblot analysis using whole-cell lysates. Annexin II, E2A-HLF, and α-tubulin proteins were detected with specific antibodies. Lanes 1 to 4: UOC-B1, YCUB-2, HAL-O1, and Endo-kun t(17;19)–positive pro-B ALL cell lines. Lanes 5 to 8: Sup-B2, RS4;11, REH, and 697 pro-B ALL cell lines without t(17;19). Lane 9: Jurkat T-ALL cell line. Lane 10: NB-4 APL cell line.