Figure 2.
Induction of annexin II by enforced expression of E2A-HLF in human ALL cells. 697 cells inducibly expressing E2A-HLF (697/E2A-HLF cells) and control 697/pMT cells were cultured in medium containing 100 μM Zn2+ for the indicated times. (Top 2 panels) Northern blot analysis of poly(A)+ RNA (1 μg). The blot was hybridized with an annexin II cDNA probe and then rehybridized with a β-actin probe. (Lower 3 panels) Immunoblot analysis detecting annexin II, E2A-HLF, and α-tubulin proteins.