Pathways regulating annexin II expression in IL-3–dependent cells. (A) β544 cells were cultured in cytokine-free medium for 8 hours (lane 2) and then in medium containing hGM-CSF for the indicated times (lanes 3-10). The Northern blot was hybridized with an annexin II cDNA probe and then rehybridized with a mouse Bcl-x_L cDNA probe. (B-C) Baf-3 cells expressing Ras^G12V (B) or Ras^G12V/V45E (C) under the regulation of Dex were cultured in IL-3–free medium containing 10^-7 M Dex in the absence (left blots) or presence (right blots) of wortmannin (0.5 μM) for the indicated times. The Northern blots were hybridized with an annexin II cDNA probe and then rehybridized with a β-actin probe. (D) FL5.12 cells inducibly expressing E2A-HLF were cultured in IL-3–free medium containing 100 μM Zn²⁺ for the indicated times. (Top 2 panels) The Northern blot was hybridized with an annexin II cDNA probe and then rehybridized with a β-actin probe. (Lower 2 panels) Immunoblot analysis to detect annexin II, E2A-HLF, and α-tubulin using specific antibodies.