Effect of E2A-HLF and annexin II on the survival of FL 5.12 cells deprived of IL-3. (A) Immunoblot analysis of 3 independently established clones of G418-resistant FL5.12 cells stably transfected with pMT/E2A-HLF (left blot) or pMT/annexin II (right blot) and control cells that received the empty pMT vector. Cells were cultured in the presence or absence of zinc. E2A-HLF (left blot, top row) and annexin II (right blot, top row) and α-tubulin (both blots, bottom row) proteins were detected using specific antibodies. (B) Survival of the transfected FL5.12 cells in the absence of IL-3. Cells growing exponentially in IL-3–containing medium in the presence or absence of 100 μM Zn2+ for 16 hours were adjusted to 5 × 105 cells/mL at hour 0, and viable cell numbers were determined at the indicated times after the removal of IL-3.
