The effect of rapamycin and dexamethasone on apoptosis in 2 MM cell lines. Karpas 707 and U-1958 cells were treated with rapamycin and/or dexamethasone for 48 hours followed by harvest and wash in PBS before staining with AV/PI and subsequent analysis by flow cytometry. Cells were categorized as living cells (AV-negative/PI-negative), apoptotic cells (AV-positive/PI-negative), apoptotic/necrotic cells (AV-positive/PI-positive), or necrotic cells (AV-negative/PI-positive), and the relative cell number was presented as the percentage of 10 000 cells. Three independent experiments per cell line were performed and one representative is shown.