American Society of Hematology

Figure 6.

$Figure 6. Gel filtration of CD81-α4β1 complexes. (A) MOLT-4 cells (5 × 106 cells) stably transduced with vector control or EWI-2–flag were surface biotinylated and lysed in 1% Brij 58. The extracts were fractionated by size exclusion chromatography on Sepharose CL6B. CD81 was immunoprecipitated from each fraction with M38 mAb, and the associated α4β1 integrin was visualized with ExtrAvidin-HRP. (B) Quantitation of results from panel A. ▦ indicates vector; □, EWI-2.$

Gel filtration of CD81-α4β1 complexes. (A) MOLT-4 cells (5 × 10⁶ cells) stably transduced with vector control or EWI-2–flag were surface biotinylated and lysed in 1% Brij 58. The extracts were fractionated by size exclusion chromatography on Sepharose CL6B. CD81 was immunoprecipitated from each fraction with M38 mAb, and the associated α4β1 integrin was visualized with ExtrAvidin-HRP. (B) Quantitation of results from panel A. ▦ indicates vector; □, EWI-2.

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